The Inverted Wide-Field Microscope is located on the sixth floor of the Medical School in room E630D. The system integrates a Zeiss Axiovert 405 microscope with software, computers and cameras. The software, Metamorph and Metafluor, is designed to support image acquisition, morphometric analysis, calcium ratio imaging, 3D reconstruction, and time lapse experiments. A recent upgrade including a Bioptechs Delta T4 stage and objective heater has greatly enhanced the live cell imaging and movie-making capabilities of the system. QuickTime and AVI movies created in Metamorph can now be exported into PowerPoint presentations. For additional information and training, please contact Maryanne Pendergast at X2575.

• Maryanne Pendergast
  Training and Facility Manager
  368-2575 (office)
  216-224-8649 (cell)
  mxp19@case.edu

• Zeiss Axiovert 405M inverted microscope.
• Mercury and Xenon arc light sources.
• Princeton Instruments Micromax cooled ccd (digital) camera.
• 35mm and 4×5 format cameras.
• Numerous objectives with long and short working distances.
• Metamorph version 4.6.6.
• Metafluor version 3.0.

• All users must be trained and approved for independent operation of the system by Maryanne.
• Everyone is expected to use and care for the equipment as demonstrated during the initial training session. Abuse of the system may result in the cancellation of user privileges; repair costs will be charged to the lab responsible for damages.
• Data can be stored short-term on the NIC server which is accessible to all computers within the facility as a mapped drive (Z:\). The server is available to other computers through a web interface. Data can also be saved to CDs and 100MB ZIP drives.
• Sign up during the day is first come/ first serve and may be restricted to two half days per week during exceptionally busy times.
• Sign-up should include user and PI names, time, and phone number.

Web site for Molecular Devices, Inc.
http://www.moleculardevices.com/pages/software/metamorph.html

Web site for Carl Zeiss, North America
http://www.zeiss.com/us/